Product Description

Gamma-enolase Monoclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB65927-50, MB65927-100

Sizes: 50µl, 100µl

Swiss-Prot: P09104

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Gamma-enolase protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Gamma-enolase expression in Hela (A), Jurkat (B), 293T (C) whole cell lysates., Immunohistochemical analysis of Gamma-enolase staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Gamma-enolase. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified