Gamma-enolase Monoclonal Antibody-MB66851

Gamma-enolase Monoclonal Antibody

Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB66851-50, MB66851-100

Sizes: 50µl, 100µl

Swiss-Prot: P09104

Host: Mouse

Reactivity: Human

Applications: WB, IHC

Background: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of Gamma-enolase protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Gamma-enolase expression in Jurkat (A), CEM (B), U251 MG (C) whole cell lysates., Immunohistochemical analysis of Gamma-enolase staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE

Immunogen: Recombinant fusion protein of human Gamma-enolase. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified