GAP43 Polyclonal Antibody-BS71926

GAP43 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71926-50, BS71926-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17677

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 43KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using GAP43 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of Neuro-2a cells using GAP43 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining., Immunofluorescence analysis of SH-SY5Y cells using GAP43 Rabbit pAb at dilution of 1:25. Blue: DAPI for nuclear staining.

Alternative Name: B-50; PP46; GAP43

Immunogen: Recombinant fusion protein of human GAP43(NP_002036.1).

Conjugate: Unconjugated

Modification: Unmodified