Product Description

GAPDH (1A6) Monoclonal Antibody-HRP

Sizes: 50µl, 100µl

Catalogue Numbers: MB001H-50, MB001H-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Mouse

Reactivity: Human, Mouse, Rat
Applications: WB,

All Applications: WB: 1:5000~20000

Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GAPDH (1A6) mAb-HRP detects endogenous levels of GAPDH protein.

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GAPDH (1A6) monoclonal antibody-HRP at 1:5000/1:10000 dilution
Lane 1: L02 whole cell lysate (20ug)
Lane 2: HEK293T whole cell lysate (20ug)
Lane 3: Panc1 whole cell lysate (20ug)
Lane 4: BV2 whole cell lysate (20ug)
Lane 5: C6 whole cell lysate (20ug)

Alternative Name: Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD

Immunogen: Recombinant full length Human GAPDH.

Conjugate: HRP

Modification: Unmodified