Product Description

GAPDH Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9231-50, MB9231-100

Product: Mouse IgM, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04406

Host: Mouse

Reactivity: Human, Mouse, Rat
Applications: WB, ICC

All Applications: WB:1:5,000-1:10,000
ICC:1:200

Background: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Purification and Purity: ProL affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GAPDH monoclonal antibody detects endogenous levels of GAPDH protein.

Bioworld Molecular Weight: 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: PC12
Lane 4: NIH/3T3
Lane 5: MCF-7
Lane 6: Rabbit liver
Lane 7: Zebrafish
, ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GAPDH, G3P, 38 kDa BFA-dependent ADP-ribosylation substrate, aging associated gene 9 protein, Aging-associated gene 9 protein, BARS-38, cb609, EC 1.2.1.12, Epididymis secretory sperm binding protein Li 162eP, G3P, G3PD, G3PDH, GAPD, GAPDH, Glyceraldehyde 3 phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase, HEL-S-162eP, KNC-NDS6, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38 component, OCT1 coactivator in S phase, 38-KD component, peptidyl cysteine S nitrosylase GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, wu:fb33a10,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified