GAPDH Polyclonal Antibody - E-AB-40337

GAPDH Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-40337-60, E-AB-40337-120, E-AB-40337-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: GAPDH

Target Synonym: aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component

Research Areas: Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q5XJ10

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Concentration: 0.66mg/mL

Immunogen: Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein

Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3.

Purification Method: Antigen Affinity Purification

Dilution: WB 1:3000-1:6000 IHC 1:300-1:500

Calculated MW: 35 kDa

Observed MW: 35 kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only