Product Description

GAPDH Polyclonal Antibody

Sizes: 50μL, 100μL, 200μL

Catalogue Numbers: E-AB-40516-50, E-AB-40516-100, E-AB-40516-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: GAPDH

Target Synonym: aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component

Research Areas: Cancer, Metabolism, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat, Zebrafish

Application: WB, IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q5XJ10

Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Concentration: 2mg/mL

Immunogen: Recombinant human GAPDH protein expressed by E.coli

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.4

Purification Method: Antigen Affinity Purification

Dilution: WB: 1:1000-2000, IF: 1:100-400

Calculated MW: 40.2kDa

Observed MW: 36kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only