GART Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90562-50, BS90562-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P22102(Human) Q64737(Mouse) Unigene:55532(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP,
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Purines are critical for energy metabolism, cell signaling and cell reproduction and also function as precursors for coenzymes, energy transfer molecules, regulatory factors and proteins involved in RNA and DNA synthesis. GART (GAR transformylase), also referred to as AIRS, GARS, PAIS, PGFT, PRGS or GARTF, is 1,010 amino acids in length and is a key folate-dependent trifunctional enzyme with phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase and AICAR (phosphoribosylaminoimidazole synthetase) activity required for de novo purine biosynthesis. Cancer cells require considerable amounts of purines to sustain their accelerated growth and GART is, therefore, a target for cancer chemotherapy. GART is highly conserved in vertebrates. Two isoforms of GART are expressed due to alternative splicing events.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GART polyclonal antibody detects endogenous levels of GART protein.
Bioworld Molecular Weight: 107 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of GART on different lysates using anti-GART antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T
Lane 3: A431, ICC staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: GARS, AIRS, GART, 5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, Glycinamide ribonucleotide synthetase, MGC47764, PAIS, PGFT, Phosphoribosyl-aminoimidazole synthetase, Phosphoribosylglycinamide formyltransferase, Phosphoribosylglycinamide formyltransferase phosphoribosylglycinamide synthetase phosphoribosylaminoimidazole synthetase, Phosphoribosylglycinamide formyltransferase, EC 2.1.2.29, Phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase, Phosphoribosylglycinamide synthetase, PRGS, PUR2, Trifunctional purine biosynthetic protein adenosine 3,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified