Product Description

GCLC Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-52359-60, E-AB-52359-120, E-AB-52359-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: GCLC

Target Synonym: EC 6.3.2.2; Gamma ECS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; Gclc; GCS; GCS heavy chain; GLCL; GLCLC; Glutamate cysteine ligase catalytic subunit; Glutamate--cysteine ligase catalytic subunit; GSH1

Research Areas: Cancer, Cell Biology, Metabolism, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P48506

Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Concentration: 0.8 mg/mL

Immunogen: Fusion protein of human GCLC

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Purification Method: Antigen affinity purification

Dilution: WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:5000-1:10000

Calculated MW: 73 kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only