GCN2 Polyclonal Antibody-BS90568

GCN2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90568-50, BS90568-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9P2K8(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The family of stress-responsive protein kinases include HRI (heme-regulated inhibitor or EIF2AK1), PKR (EIF2AK2 or TIK), PERK (EIF2AK3) and GCN2 (EIF2AK4). These proteins phosphorylate the eukaryotic translation initiation factor 2α (eIF2α) on Ser 51 to regulate general and gene-specific protein synthesis. Phosphorylated eIF2α acts as an inhibitor of its guanine nucleotide exchange factor eIF2B. GCN2, a unique eIF2α kinase, exists in all eukaryotes from yeast to mammals. In mammals, expression of GCN2 is highest in liver and brain tissues. GCN2 primarily initiates the phosphorylation of eIF2α in response to UV, but has been shown to increase phosphorylation activity in response to serum starvation. Also, substitution of Asp 83 for Ala on eIF2α results in impaired phosphorylation by GCN2 and PKR, suggesting a contribution of remote residues to kinase-substrate recognition.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GCN2 polyclonal antibody detects endogenous levels of GCN2 protein.

Bioworld Molecular Weight: 187 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Fig1:ICC staining GCN2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining GCN2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: EIF2AK4, GCN2, KIAA1338, E2AK4, Eif2ak4, Eukaryotic Translation Initiation Factor 2 alpha kinase 4, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2, GCN2 eIF2alpha kinase, GCN2 like protein, GCN2-like protein, KIAA1338, MGCN2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified