Product Description

GCSc-γ (E315) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS2926-50, BS2926-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The GCLC gene consists of 16 exons and encodes the 636 amino acid protein γ-GCSc (γ-glutamylcysteine synthetase heavy subunit), also designated γ-Lglutamate- L-cysteine ligase catalytic subunit (GLCLC). γ-GCSc is expressed in hemocytes, brain, liver and kidney. γ-GCSc associates with a regμlatory or modifier subunit, γ-GCSm (γ-glutamylcysteine synthetase light subunit), to form a heterodimer, γ-GCS. γ-GCS is the first enzyme involved and the rate determining step in glutathione iosynthesis. Oxidants, cadium and methyl mercury upregμlate the transcription of γ-GCS. H2O2 regμlation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regμlates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of γ-GCS.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Glutamate--cysteine ligase catalytic subunit, 6.3.2.2, GCS heavy chain, Gamma-ECSGamma-glutamylcysteine synthetase, GLCL, GLCLC, GCLC

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human GCSc-γ.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GCSc-γ (E315) polyclonal antibody detects endogenous levels of GCSc-γ protein.

Molecular Weight: ~ 70 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P48506

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only