GCSc-γ (E315) Polyclonal Antibody-BS2926

GCSc-γ (E315) Polyclonal Antibody

Catalogue Numbers: BS2926-50, BS2926-100

Sizes: 50µl, 100µl

Alternative Name: Glutamate--cysteine ligase catalytic subunit; 6.3.2.2; GCS heavy chain; Gamma-ECS
Gamma-glutamylcysteine synthetase; GLCL; GLCLC; GCLC

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P48506

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The GCLC gene consists of 16 exons and encodes the 636 amino acid protein γ-GCSc (γ-glutamylcysteine synthetase heavy subunit), also designated γ-Lglutamate- L-cysteine ligase catalytic subunit (GLCLC). γ-GCSc is expressed in hemocytes, brain, liver and kidney. γ-GCSc associates with a regulatory or modifier subunit, γ-GCSm (γ-glutamylcysteine synthetase light subunit), to form a heterodimer, γ-GCS. γ-GCS is the first enzyme involved and the rate determining step in glutathione iosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of γ-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of γ-GCS.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GCSc-γ (E315) polyclonal antibody detects endogenous levels of GCSc-γ protein.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GCSc-γ (E315) polyclonal antibody at 1:500 dilution
Lane 1: HepG2 whole cell lysate(40ug)
Lane 2: NIH-3T3 whole cell lysate(40ug)
Lane 3: PC12 whole cell lysate(40ug)
Lane 4: A549 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human GCSc-γ.

Conjugate: Unconjugated

Modification: Unmodified