Product Description

GCSm-γ (V91) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2927-50, BS2927-100

Sizes: 50µl, 100µl

Swiss-Prot: P48507

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Gamma-glutamylcysteine synthetase (γ-GCS) is the rate limiting enzyme for glutathione (L-γ-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. γ-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic ntioxidants, heat shock, oxidants and cytokines. The human γ-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human γ-GCS gene encoding the regulatory subunit maps to chromosome 1p22-p21. The two subunits of γ-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GCSm-γ (V91) polyclonal antibody detects endogenous levels of GCSm-γ protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GCSm-γ (V91) pAb at 1:500 dilution
Lane 1: MEF whole cell lysate (40ug)
Lane 2: The Spleen tissue lysate of Mouse (40ug)
Lane 3: HEK293T whole cell lysate (40ug)
Lane 4: Panc1 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of GCSm-γ (V91) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 30 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Glutamate--cysteine ligase regulatory subunit; GCS light chain; Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; Glutamate--cysteine ligase modifier subunit; GLCLR; GCLM

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human GCSm-γ.

Conjugate: Unconjugated

Modification: Unmodified