GDNF Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90569-50, BS90569-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P39905(Human) P48540(Mouse) Q07731(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, FC
All Applications: WB:1:500-1:1,000
FC:1:50-1:200
ICC:1:50-1:200
IHC:1:50-1:200
Background: Glial cell line-derived neurotrophic factor (GDNF) has been identified as a potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons. GDNF is a glycosylated, disulfide-bonded homodimer and is a distantly related member of the TGFβ superfamily of growth regulatory ligands. GDNF contains the seven conserved cysteine residues in the same relative spacing characteristic of all members of the TGFβ superfamily. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. On the basis of these findings, it has been suggested that GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GDNF polyclonal antibody detects endogenous levels of GDNF protein.
Bioworld Molecular Weight: 24 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of GDNF on HepG2 cell using anti-GDNF antibody at 1/1,000 dilution., Flow cytometric analysis of SH-SY5Y cells with GDNF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Alternative Name: hGDNF, ATF, GDNF, Astrocyte derived trophic factor, Astrocyte derived trophic factor 1, Astrocyte-derived trophic factor, ATF 1, ATF 2, Atf, ATF1, ATF2, gdnf, GDNF, Glial cell derived neurotrophic factor, Glial Cell Line Derived Neurotrophic Factor, Glial cell line-derived neurotrophic factor, Glial derived neurotrophic factor, HFB1 GDNF, hGDNF, HSCR3,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified