Gelsolin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90571-50, BS90571-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P06396(Human) P13020(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC, IF, IHC, FC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Gelsolin (also known as brevin, Actin-depolymerizing factor or ADF), a protein of leukocytes, platelets and other cells, severs Actin filaments in the presence of submicromolar calcium, thereby isolating cytoplasmic Actin gels. A calcium-independent mechanism reverses the process. A Gelsolin variant with 23 more amino-terminal amino acids is a plasma component probably involved in the clearance of Actin, the most abundant human protein, from the circulation. It has been suggested that a single gene encodes both cell and plasma gelsolins. Gelsolin may be unique in that it is made for both secretion and intracytoplasmic location. Amino acid homology was identified between Gelsolin and the amyloid of the Finnish variety of amyloidosis. The amyloid in this disorder is antigenically and structurally related to Gelsolin. Gelsolin is the principal intracellular and extracellular Actin-severing protein. Gelsolin and Gc protein together constitute the extracellular Actin-scavenger system which prevents the toxic effects of Actin release into the extracellular space under circumstances of cell necrosis.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Gelsolin polyclonal antibody detects endogenous levels of Gelsolin protein.
Bioworld Molecular Weight: 86 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse thymus
Lane 2: Mouse lung
Lane 3: THP-1
Lane 4: HepG2, ICC staining Gelsolin in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: ADF, GSN, Actin depolymerizing factor, Actin-depolymerizing factor, ADF, AGEL, Brevin, DKFZp313L0718, GELS, Gelsolin, Gsn,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified