Product Description

GFAP Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9017-50, MB9017-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14136

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHCICCFC

All Applications: WB: 1:2000-1:5000 IHC/ICC:1:200
FC: 1:200

Background: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of GFAP and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GFAP Mouse mAb at 1:5000 dilution
Lane 1: The brain tissue lysate of Rat
Lane 2: The brain tissue lysate of Human, Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAPantibody. Counter stained with hematoxylin., ICC staining of GFAP in A172 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Glial fibrillary acidic protein

Immunogen: This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of GFAP.

Conjugate: Unconjugated

Modification: Unmodified