Product Description

GFAP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6460-50, BS6460-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P14136

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GFAP pAb at 1:1000 dilution
Lane 1: U-87MG whole cell lysate (30ug)
Lane 2: SHSY5Y whole cell lysate (30ug)
Lane 3: EC9706 whole cell lysate (30ug)
Lane 4: 3T3-L1 whole cell lysate (30ug)
Lane 5: C6 whole cell lysate (30ug), Immunohistochemistry of paraffin-embedded rat brain using GFAP antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat cerebellum using GFAP antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using GFAP antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: ALXDRD; GFAP

Immunogen: A synthetic peptide of human GFAP(NP_002046.1).

Conjugate: Unconjugated

Modification: Unmodified