Product Description

GFI1B Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS60592-50, BS60592-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Growth factor independent 1 (Gfi-1) is a transcriptional repressor that spe-cifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resμlting in the inhibition of cell death. Gfi-1 is expressed outside the lymphoid system in granμlocytes and activated macrophages. Gfi-1B, a related protein, is a transciptional repressor primarily expressed in bone marrow and spleen. Gfi-1B is a direct repressor of the p21 promoter and the SOCS-1 and -3 promoters. The genes encoding human Gfi-1 and Gfi-1B map to chromosome 1p22 and 9q34.13, respectively.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Zinc finger protein Gfi-1b, Growth factor independent protein 1B, Potential regμlator of CDKN1A translocated in CML, GFI1B

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: A synthetic peptide corresponding to residues in Human GFI1B

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GFI1B polyclonal antibody detects endogenous levels of GFI1B protein.

Molecular Weight: ~ 37 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q5VTD8

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only