GFP Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9233-50, MB9233-100
Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P42212
Host: Mouse
Applications: WB, ICC ELISA IP, FC
All Applications: WB:1:2,000
ICC:1:200
Background: Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GFP monoclonal antibody detects endogenous levels of GFP protein.
Bioworld Molecular Weight: 26 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of GFP on transfected K562 with GFP using anti-GFP antibody at 1/2000 dilution., ICC staining GFP in transfected K562 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: GFP, GFP, Green fluorescent protein, yfp,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified