Product Description

GHRHR Polyclonal Antibody

Catalogue Numbers: BS5732-50, BS5732-100

Sizes: 50µl, 100µl

Alternative Name: Growth hormone-releasing hormone receptor; GHRH receptor; Growth hormone-releasing factor receptor; GRF receptor; GRFR; GHRH-R

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q02643

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: GHRH-R (growth hormone-releasing hormone receptor) is a seven transmembrane domain protein that localizes to the somatotroph of the pituitary. GHRH-R plays an important role in growth and acts as a high-affinity receptor for GHRH. Binding of GHRH leads to the coupling of GHRH-R to G-protein which stimulates increased adenylyl cyclase activity and the accumulation of cAMP leading to the synthesis and release of growth hormone and somatotroph proliferation. In addition, this signalling pathway may have direct action in fetal/placental development, reproduction and immune function. GHRH and GHRH-R may also play a role in the regulation of non-rapid eye movement sleep (NREMS). The expression of GHRH-R is dependent on the presence of the POU domain factor Pit-1. Mutations in the gene encoding this protein can result in isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, and anterior pituitary hypoplasia (APH).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GHRHR polyclonal antibody detects endogenous levels of GHRHR protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GHRHR polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 378-423 of Human GHRHR.

Conjugate: Unconjugated

Modification: Unmodified