Product Description

GIP Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS7977-50, BS7977-100

Swiss-Prot: P09681

Host: Rabbit

GIP Polyclonal Antibody-BS7977

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, ELISA IHC, IF ICC

All Applications: WB: 1:500-1:2000
ELISA: 1:5000-1:10000
IHC: 1:100-1:500
IF: 1:100-1:500
ICC: 1:100-1:500

Background: Glucose-dependent insulinotropic polypeptide (GIP) is a major physiologic factor in the augmentation of the insulin response to oral glucose. GIP is a peptide hormone that is released postprandially from the small intestine and acts in concert with glucagon-like peptide (GLP)-1 to potentiate glucose-induced insulin secretion from the pancreatic β-cell. GIP has been shown to increase adenylyl cyclase activity, elevate intracellular calcium levels, and stimulate a mitogen-activated protein kinase pathway in the pancreatic β-cell. Additionally, nutrient protein provides a potent stimulus for GIP expression, an effect that occurs at the posttranslational level and may be mediated in part through the acid-stimulatory properties of protein. GIP release is demonstrated predominantly after ingestion of carbohydrate and fat and the effects of acid on GIP are consistent with a role for GIP as an enterogastrone.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GIP polyclonal antibody detects endogenous levels of GIP protein.

Bioworld Molecular Weight: ~ 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GIP) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (GIP) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Alternative Name: Gastric Inhibitory polypeptide; Gastric inhibitory polypeptide precursor; GIP; Glucose dependent insulinotropic polypeptide; Gastric Inhibitory Peptide; GIP_HUMAN; Glucose-dependent insulinotropic polypeptide; Incretin hormone.

Immunogen: KLH conjugated synthetic peptide derived from human GIP:52-93/153.

Modification: Unmodified

Conjugate: Unconjugated