GIT1 Polyclonal Antibody-BS90574

GIT1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90574-50, BS90574-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y2X7(Human) Q68FF6(Mouse) Q9Z272(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC FC

All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade. GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking. GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: GIT1 polyclonal antibody detects endogenous levels of GIT1 protein.

Bioworld Molecular Weight: 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GIT1 antibody. Counter stained with hematoxylin.

Alternative Name: ARF GAP GIT1, CAT-1, CAT1, GIT1, ARF GAP GIT1, ARF GTPase activating protein GIT1, ARF GTPase-activating protein GIT1, CAT 1, CAT-1, CaT1, Cool associated and tyrosine phosphorylated protein 1, Cool-associated and tyrosine-phosphorylated protein 1, G protein coupled receptor kinase interacting ArfGAP 1, G protein coupled receptor kinase interactor 1, G protein-coupled receptor kinase-interactor 1, GIT1, GIT1, GRK interacting protein 1, GRK-interacting protein 1,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified