GLB1 Polyclonal Antibody
Catalogue Numbers: BS60421-50, BS60421-100
Sizes: 50µl, 100µl
Alternative Name: Beta-galactosidase; Acid beta-galactosidase; Lactase; Elastin receptor 1; GLB1; ELNR1
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P16278
Host: Rabbit
Reactivity: Human, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The human b-galactosidase gene, known as the LacZ gene, maps to chromosome 3p21.33 and encodes a 677 amino acid protein with an optimum functional pH range of 6 to 8. Catalytically active b-galactosidases (b-Gal) is a tetramer of four identical subunits, each with an active site, which can independently catalyze the cleavage of terminal galactose. Monovalent cations have a stimulatory effect on the enzymatic reaction, which likely involves a galactosyl-enzyme complex intermediate. b-Gals are widespread in animals, microorganisms and plants. The LacZ gene is widely used as a reporter gene with a variety of colored or fluorescent compounds capable of being produced from appropriate substrates, such as Xgal, which produces a blue color. For this reason, LacZ is incorporated into numerous plasmid vectors as a marker.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: GLB1 polyclonal antibody detects endogenous levels of GLB1 protein.
Bioworld Molecular Weight: ~ 90 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of GLB1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human GLB1.
Conjugate: Unconjugated
Modification: Unmodified