Product Description

Gli1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90575-50, BS90575-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08151(Human)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC

All Applications: WB:1:500-1:1,000
IHC:1:50-1:200
ICC:1:50-1:200

Background: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. GLI-1 (GLI family zinc finger 1), also known as Glioma-associated oncogene or oncogene GLI, is a 1,106 amino acid protein that localizes to both the cytoplasm and nucleus, and belongs to the GLI C2H2-type zinc-finger protein family. GLI-1 acts as a transcriptional activator and is thought to play a role in craniofacial development. GLI-1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 12q13.3.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Gli1 polyclonal antibody detects endogenous levels of Gli1 protein.

Bioworld Molecular Weight: 150 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Gli1 on SH-SY-5Y cells lysates using anti-Gli1 antibody at 1/1,000 dilution., ICC staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: GLI1, Gli 1, GLI, GLI family zinc finger 1, GLI Kruppel family member 1, gli1, GLI1, Glioma associated oncogene 1, Glioma associated oncogene homolog 1 (zinc finger protein), Glioma associated oncogene homolog, Glioma-associated oncogene, Oncogene GLI, Zfp 5, Zfp5, Zinc finger protein GLI 1, Zinc finger protein GLI1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified