GLO1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90577-50, BS90577-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q04760(Human) Q9CPU0(Mouse) Q6P7Q4(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500-1:2,000
ICC:1:100-1:500
IHC:1:100-1:500
FC:1:50-1:100
Background: The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP). Glyoxalase I (GLO1), a member of the glyoxalase family, is effective in eliminating GDP. Overexpression or silencing of Glyoxalase I in mice brain suggests an association between Glyoxalase I and anxiety. Glyoxalase I has three isoforms generated from two alleles in the genome which forms two homodimers and one heterodimer, each subunit binding one zinc ion. Research demonstrates that GLO1 gene expression is induced in colon carcinoma. Both an insulin response element (IRE), and a zinc metal response element (MRE) in the promoter region of the GLO1 gene have been identified.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GLO1 polyclonal antibody detects endogenous levels of GLO1 protein.
Bioworld Molecular Weight: 21 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of GLO1 on different lysates using anti-GLO1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse testis tissue
Lane 2: C2C12
Lane 3: 293
Lane 4: Rat spleen tissue, ICC staining GLO1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Glx I, GLO1, Aldoketomutase, glo1, GLOD1, Glx I, GLYI, glyoxalase domain containing 1, Glyoxalase I, Ketone aldehyde mutase, Ketone-aldehyde mutase, Lactoyl glutathione lyase, Lactoylglutathione lyase, LGUL, Methylglyoxalase, S D lactoylglutathione methylglyoxal lyase, S-D-lactoylglutathione methylglyoxal lyase,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified