
Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity Assay Kit
Size: 96T
Catalogue Number: E-BC-K056-M-96
Citations, Manuals and MSDS Available upon request.
Target Synonym: G-6-PD;G6PDH
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Enzyme Activity
Detection Instrument: Microplate reader
Tested Sample Types: Serum, plasma, animal tissue
Assay Time: 20 min
Detection Type: Colorimetric method
Sensitivity: 0.01 U/L
Detection Range: 0.01-50 U/L
Test Principle: Under the presence of G6PDH, glucose-6-phosphoric acid is oxidized to 6-PG. In this reaction, NADP+ is reduced to NADPH. Under the action of electron coupling reagent 1-MPMS, NADPH reducts wST-8 to form orange formazan, which has the maximum absorption peak at about 450 nm. Formazan generated in the reaction system is proportional to the activity of G6DPH in the sample.
Inter CV: 5.8
Intra CV: 2.1
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag