Product Description

Glucose-6-phosphate (G6P) Colorimetric Assay Kit

Size: 96T

Catalogue Number: E-BC-K011-M-96

Citations, Manuals and MSDS Available upon request.

Target Synonym: G6P

Research Areas: Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: Serum, plasma, animal tissue

Assay Time: 35 min

Detection Type: Colorimetric method

Sensitivity: 5.6 μmol /L

Detection Range: 5.6-500 μmol/L

Test Principle: Glucose-6-phosphate (G6P) is a molecule generated by phosphorylation of hydroxyl groups on the sixth carbon of glucose under the catalysis of hexokinase. It is a common small molecule of sugar metabolism in cells and participates in biochemical pathways such as glycolysis and pentose phosphate pathway. In the first reaction of glycolysis, glucose is catalyzed by hexokinase to produce glucose-6-phosphate, which is then catalyzed by phosphoglucose isomerase to form fructose-6-phosphate to continue the other steps of glycolysis: In the pentose phosphate pathway, glucose-6-phosphate is the first substrate, and this process is also the main way to generate NADPH. In addition to these two metabolic pathways, glucose-6-phosphate can also be converted into glycogen or starch and stored.

Inter CV: 4.3

Intra CV: 2.1

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag