Glucosylceramidase beta (GBA) Polyclonal Antibody-BS71215

Glucosylceramidase beta (GBA) Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71215-50, BS71215-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04062

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of MCF-7 cells, using Glucosylceramidase beta ) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 60kDa

Specificity: Polyclonal Antibodies

Alternative Name: GBA; GBA1; GCB; GLUC

Immunogen: Recombinant fusion protein of human Glucosylceramidase beta (Glucosylceramidase beta (GBA))(NP_000148.2).

Conjugate: Unconjugated

Modification: Unmodified