Glutaminase Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90586-50, BS90586-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O94925(Human) D3Z7P3(Mouse) P13264(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: Glutamine is an important molecule involved in several cellular functions, including nitrogen and carbon transport, hepatic urea synthesis, renal ammoniagenesis, and gluconeogenesis. Glutamine is catabolized by either the liver-type (LGA) or kidney-type (KGA) glutaminase. KGA is mitochondrial specific protein whose expression in kidney is increased during metabolic acidosis. This process is mediated by an 8-base AU-sequence in KGA that functions as a pH-response element. The human KGA gene maps to chromosome 2, and produces three isoforms, designated KGA, GAC, and GAM, by alternative splicing. KGA is synthesized as a cytosolic protein that is transported to the mitochondria as an intermediate protein, and is further cleaved into the KGA isoform and the GAC isoform. The processing of the GAM isoform is unclear. The KGA isoform is abundant in brain and kidney, while the GAC isoform is principally expressed in cardiac muscle and pancreas. The GAM isoform is solely expressed in cardiac and skeletal muscle.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Glutaminase polyclonal antibody detects endogenous levels of Glutaminase protein.
Bioworld Molecular Weight: 65 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Glutaminase on different lysates using anti-Glutaminase antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293, ICC staining Glutaminase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: GLS, AAD20, DKFZp686O15119, FLJ10358, GAC, GAM, GLS, GLS1, GLSK, Glutaminase C, Glutaminase kidney isoform, Glutaminase phosphate activated, K-glutaminase, KGA, KIAA0838, L-glutamine amidohydrolase, mitochondrial,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified