Glycogen Fluorometric Assay Kit
Sizes: 96T, 500 Assays
Catalogue Numbers: E-BC-F040-96, E-BC-F040-500
Citations, Manuals and MSDS Available upon request.
Research Areas: Glycolysis and Lipid Metabolism
Result Type: Quantitative
Detection Instrument: Fluorescence microplate reader
Tested Sample Types: Animal liver and muscle tissue
Assay Time: 50min
Detection Type: Fluorescence method
Sensitivity: 0.06 μg/mL
Detection Range: 0.06-4.0 μg/mL
Test Principle: Glycogen produces glucose under the action of starch glycosidase, and glucose is catalyzed by glucose oxidase to produce hydrogen peroxide. In the presence of the peroxidase, hydrogen peroxide be oxidized to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the glycogen content.
Inter CV: 6.6
Intra CV: 3.4
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag