Product Description

GLYR1 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71554-50, BS71554-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q49A26

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:100 - 1:200

Background: Nucleosome-destabilizing factor that is recruited to genes during transcriptional activation. Facilitates Pol II transcription through nucleosomes. Binds DNA (in vitro. Recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3. Promotes KDM1B demethylase activity. Stimulates the acetylation of 'Lys-56' of nucleosomal histone H3 (H3K56ac by EP300. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Putative oxidoreductase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using GLYR1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Bioworld Molecular Weight: 60kDa

Specificity: Polyclonal Antibodies

Alternative Name: GLYR1; BM045; HIBDL; N-PAC; NP60

Immunogen: Recombinant fusion protein of human GLYR1(NP_115958.2).

Conjugate: Unconjugated

Modification: Unmodified