GM13125 Polyclonal Antibody-BS74944

GM13125 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS74944-50, BS74944-100

Swiss-Prot: Q5VT98

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Predicted to enable ubiquitin ligase-substrate adaptor activity. Predicted to be part of Cul2-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. Orthologous to several human genes including PRAMEF1 (PRAME family member 1); PRAMEF10 (PRAME family member 10); and PRAMEF11 (PRAME family member 11).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 55kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using GM13125 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

Alternative Name: PRAMEF20; PRAMEF21; Gm13125

Immunogen: Recombinant fusion protein of mouse GM13125 (NP_001108549.1).

Conjugate: Unconjugated

Modification: Unmodified