Product Description

GNAI1 Polyclonal Antibody

Catalogue Numbers: BS60415-50, BS60415-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P63096

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein α, β and γ polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their α subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gα subunits have been identified; these include Gs, Gi, Gq and Gα 12/13. The Gi class comprises all the known α subunits that are susceptible to pertussis toxin modifications, including Gα i-1, Gα i-2, Gα i-3, Gα o, Gα t1, Gα t2, Gα z and Gα gust. Of these, the three Gα i subtypes function to open atrial potassium channels.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GNAI1 polyclonal antibody detects endogenous levels of GNAI1 protein.

Extra Notes: Western blot (WB) analysis of GNAI1 pAb at 1:500 dilution
Lane1:C6 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:Myla205.9 whole cell lysate(40ug)
Lane4:The brain tissue lysate of Rat(20ug)
Lane5:The brain tissue lysate of Mouse(40ug)

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Guanine nucleotide-binding protein G(i) subunit alpha-1; Adenylate cyclase-inhibiting G alpha protein; Gα i-1

Immunogen: A synthetic peptide corresponding to residues in Human GNAI1.

Conjugate: Unconjugated

Modification: Unmodified