Product Description

GNL1 (Q103) Polyclonal Antibody

Catalogue Numbers: BS3312-50, BS3312-100

Sizes: 50µl, 100µl

Alternative Name: Guanine nucleotide-binding protein-like 1; GTP-binding protein HSR1; GNL1; HSR1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P36915

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: GNL1 (guanine nucleotide-binding protein-like 1) is a nuclear protein that likely acts as a regulator of the histocompatibility cluster. GNL1 and MMR1, the murine homolog, are localized within or close to the MHC class I region and belong to the MMR1/HSR1 GTP-binding protein family. GTPases, such as GNL1, from the MMR1/HSR1 GTP-binding protein subfamily are circularly rearranged G-motifs that play a critical role in maintaining normal cell growth. Deletion of these genes results in severe growth defects with a marked reduction in mature rRNA species and a concomitant accumulation of the 35S pre-rRNA transcript. Deletion also causes the ribosomal protein RPL25A to fail exportation from the nucleolus. Deletion of any of the G-domain motifs will result in a null phenotype and nuclear/nucleolar localization that lacks the nucleolar export of preribosomes and is accompanied by a distortion of the nucleolar structure. Upon DNA damage GNL1 is phosphorylated by a kinase, possibly Atm or ATR.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GNL1 (Q103) polyclonal antibody detects endogenous levels of GNL1 protein.

Bioworld Molecular Weight: ~ 69 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GNL1 (Q103) pAb at 1:1000 dilution
Lane 1: The Brain tissue lysate of Mouse(40ug)
Lane 2: The Brain tissue lysate of Rat(40ug)
Lane 3: HEK293T whole cell lysate(40ug)
Lane 4: MCF-7 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 80-120 of Human GNL1.

Conjugate: Unconjugated

Modification: Unmodified