GNL3 Polyclonal Antibody-BS8233

GNL3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8233-50, BS8233-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BVP2

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100 | IP,1:50 - 1:100

Background: The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 62kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using GNL3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of MCF7 cells using GNL3 antibody. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug GNL3 antibody. Western blot was performed from the immunoprecipitate using GNL3 antibody at a dilution of 1:500.

Alternative Name: GNL3; C77032; E2IG3; NNP47; NS

Immunogen: Recombinant fusion protein of human GNL3(NP_996562.1).

Conjugate: Unconjugated

Modification: Unmodified