Product Description

Goat Anti-Rabbit IgG (H&L) Highly Cross-Adsorbed - AF488

Sizes: 100μl, 500μl

Catalogue Numbers: BS22352-100, BS22352-500

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG. No antibody was detected against non immunoglobμlin serum proteins.

Description: Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) Highly Cross-Adsorbed and conjugate to AF488

Category: Secondary Antibody

Concentration: 1 mg/ml

Label: AF488

Host: Goat

Immunogen: Rabbit IgG

Applications: E, IH, IF, FC

Dilution: IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)

Buffer: 1 mg/ml, liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide

Preservative: 0.02% Proclin/Sodium Azide

Purification: Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, guinea pig, hamster, horse, mouse, rat, and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resμlting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns resμlt in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in mμltiplexing/mμlticolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobμlins.

Storage and Stability: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiration Date: Six months from date of reconstitution.

Research Use Only