GOLPH3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90589-50, BS90589-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H4A6(Human) Q9CRA5(Mouse) Q9ERE4(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC FC
All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: GOLPH3 (golgi phosphoprotein 3), also known as GOPP1, GPP34 or MIDAS, is a 298 amino acid protein that localizes to both the cytoplasm and the Golgi stack membrane where it is thought to play a regulatory role in protein trafficking within the Golgi. GOLPH3 is subject to post-translational phosphorylation and is encoded by a gene which maps to human chromosome 5. Chromosome 5 contains 181 million base pairs and comprises nearly 6% of the human genome. Chromosome 5 is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene. Treacher Collins syndrome is also chromosome 5-associated and is caused by insertions or deletions within the TCOF1 gene. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GOLPH3 polyclonal antibody detects endogenous levels of GOLPH3 protein.
Bioworld Molecular Weight: 34 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining GOLPH3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining GOLPH3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: MIDAS, GOLPH3, GPP34, Coat protein, Coat protein GPP34, FLJ90675, Golgi associated protein, Golgi peripheral membrane protein 1, 34 kDa, Golgi phosphoprotein 3 (coat protein), Golgi phosphoprotein 3, Golgi protein, GOLP3, Golph3, GOPP1, GPP34, MIDAS, Mitochondrial DNA absence factor,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified