Product Description

gp91-phox/NOX2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90591-50, BS90591-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04839(Human) Q61093(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:500-1:1,000
FC:1:50-1:100

Background: Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91-phox is expressed in eosinophils, neutro-phils, monocytes and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Mox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Mox1 may function analogously to gp91-phox, yet regulate the NADPH superoxide production in non-phagocytic cells.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: gp91-phox/NOX2 polyclonal antibody detects endogenous levels of gp91-phox/NOX2 protein.

Bioworld Molecular Weight: 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of NOX2/gp91phox on A549 cell lysates using anti-NOX2/gp91phox antibody at 1/500 dilution., ICC staining NOX2/gp91phox in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Cytochrome b558 subunit beta, CYBB, NOX2, AMCBX2, CGD, CGD91-phox, CY24B, CYBB, Cytochrome b 245, beta polypeptide, Cytochrome b(558) beta chain, Cytochrome b(558) subunit beta, Cytochrome b-245 heavy chain, Cytochrome b558 subunit beta, GP91 PHOX, gp91-1, gp91-phox, GP91PHOX, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, NOX2, p22 phagocyte B-cytochrome, P91 PHOX, p91-PHOX, Superoxide-generating NADPH oxidase heavy chain subunit,

Immunogen: Synthetic peptide corresponding to C-terminal human NOX2/gp91phox.

Conjugate: Unconjugated

Modification: Unmodified