Product Description

GPAA1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75492-50, BS75492-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43292

Host: Rabbit

Reactivity: Mouse

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of mouse heart, using GPAA1 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 68kDa

Alternative Name: GPAA1; GAA1; hGAA1

Immunogen: Recombinant fusion protein of human GPAA1(NP_003792.1).

Conjugate: Unconjugated

Modification: Unmodified