Product Description

GPR105 Polyclonal Antibody

Catalogue Numbers: BS5738-50, BS5738-100

Sizes: 50µl, 100µl

Alternative Name: P2Y purinoceptor 14; P2Y14; G-protein coupled receptor 105; UDP-glucose receptor; P2RY14; KIAA000

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15391

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR105, also designated P2Y14, is widely expressed throughout many brain regions where it localizes to glial cells, and specifically co-localizes with astrocytes. GPR105 is upregulated when a tissue is immunologically challenged with lipopolysaccharide, leading to the theory that GPR105 may play an important role in modulating peripheral and neuroimmune function.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GPR105 polyclonal antibody detects endogenous levels of GPR105 protein.

Bioworld Molecular Weight: ~ 39 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GPR105 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: Rat kidney tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 197-242 of Human GPR105.

Conjugate: Unconjugated

Modification: Unmodified