Product Description

GPR12 Polyclonal Antibody

Catalogue Numbers: BS5739-50, BS5739-100

Sizes: 50µl, 100µl

Alternative Name: G-protein coupled receptor 12

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P47775

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: G protein-coupled receptors (GPRs) are a protein family of transmembrane receptors that transmit an extracellular signal (ligand binding) into an intracellular signal (G protein activation). GPR signaling is an evolutionarily ancient mechanism used by all eukaryotes to sense environmental stimuli and mediate cell-cell communication. All of the receptors have seven membrane-spanning domains and the extracellular parts of the receptor can be glycosylated. These extracellular loops also contain two highly conserved cysteine residues which create disulfide bonds to stabilize the receptor structure. GPR12 is a 334 amino acid peptide that is expressed primarily in brain, particularly in regions where neuronal differentiation takes place. GPR12 is coupled to an inhibitory G protein. It positively influences differentiation and maturation of post-mitotic neurons, and it may promote the growth of neuronal precursor cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GPR12 polyclonal antibody detects endogenous levels of GPR12 protein.

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GPR12 polyclonal antibody at 1:500 dilution
Lane 1: HepG2 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: Rat heart tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 51-96 of Human GPR12.

Conjugate: Unconjugated

Modification: Unmodified