Product Description

GPR124 Polyclonal Antibody

Catalogue Numbers: BS62571-50, BS62571-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96PE1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex. ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly. Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GPR124 polyclonal antibody detects endogenous levels of GPR124 protein.

Extra Notes: Western blot (WB) analysis of GPR124 polyclonal antibody at 1:500 dilution
LaneA:CT26 whole cell lysate
LaneB:PMVEC whole cell lysate
LaneC:MEF whole cell lysate
LaneD:A549 whole cell lysate
LaneE:LOVO whole cell lysate

Bioworld Molecular Weight: ~ 142 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Adhesion G protein-coupled receptor A2; G-protein coupled receptor 124; Tumor endothelial marker 5; ADGRA2; GPR124; KIAA1531; TEM5

Immunogen: Synthetic peptide, corresponding to Human GPR124.

Conjugate: Unconjugated

Modification: Unmodified