Product Description

GPR146 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67492-50, BS67492-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q96CH1

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)

Background: G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR146 (G protein-coupled receptor 146), also known as PGR8, is a 333 amino acid multi-pass transmembrane protein that belongs to the G-protein coupled receptor 1 family. Characterized as an orphan receptor for which its endogenous ligand has yet to be identified, GPR146 is thought to play a role in signaling events throughout the cell.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GPR146 protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GPR146 expression in A549 (A), Hela (B), rat lung (C) whole cell lysates., Immunofluorescent analysis of GPR146 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: PGR8; Probable G-protein coupled receptor 146; G-protein coupled receptor PGR8

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR146. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified