Product Description

GPR38 Polyclonal Antibody

Catalogue Numbers: BS5749-50, BS5749-100

Sizes: 50µl, 100µl

Alternative Name: Motilin receptor; G-protein coupled receptor 38; MLNR; GPR38; MTLR; MTLR1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43193

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Motilin is a widely conserved 22 amino acid peptide hormone secreted from enterochromaffin cells of the gastrointestinal tract. Within the intestines, motilin potentiates intestinal motility by inducing contractions of the duodenum and through binding to surface receptors, designated GPR38 (G-protein coupled receptor 38). These motilin receptors are predominantly expressed in stomach, thyroid and bone marrow, and they are related to other G-protein coupled receptors located in the pituitary and hypothalamus, which mediate the signaling for growth hormone secretagogues. The gene encoding GPR38 is alternately spliced at the carboxy terminus to generate two related proteins that are designated GPR38-A and GPR38-B. Sequence comparisons of the two isoforms indicate that GPR38-A contains seven transmembrane domains while GPR38-B is predicted to contains only five transmembrane regions. Consistent with other G-protein coupled receptors, GPR38 activates phospholipase C signal transduction pathways and induces intracellular calcium mobilization after binding of motilin.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GPR38 polyclonal antibody detects endogenous levels of GPR38 protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GPR38 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: NIH-3T3 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 367-412 of Human GPR38.

Conjugate: Unconjugated

Modification: Unmodified