Product Description

GPR42 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67497-50, BS67497-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: O15529

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)

Background: Probable G protein-coupled receptor that may be activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that may play a role in the regulation of whole-body energy homeostasis and/or in intestinal immunity.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GPR42 protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GPR42 expression in HCT116 (A), U87MG (B), rat brain (C), mouse brain (D) whole cell lysates., Immunofluorescent analysis of GPR42 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: GPR42P; G-protein coupled receptor 42

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR42. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified