Product Description

GPR75 Polyclonal Antibody

Catalogue Numbers: BS5752-50, BS5752-100

Sizes: 50µl, 100µl

Alternative Name: Probable G-protein coupled receptor 75

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95800

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR75 is a 540 amino acid multi-pass membrane protein that functions as an orphan receptor and belongs to the GPR1 family. Highly expressed in spinal cord and brain, GPR75 is also found at low levels in retinal pigment epithelium. The gene encoding GPR75 maps to human chromosome 2, which consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2 including Harlequin icthyosis, sitosterolemia and Alström syndrome.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GPR75 polyclonal antibody detects endogenous levels of GPR75 protein.

Bioworld Molecular Weight: ~ 59 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GPR75 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: Rat kidney tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 423-468 of Human GPR75.

Conjugate: Unconjugated

Modification: Unmodified