Product Description

GPS2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71600-50, BS71600-100

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IP

All Applications: WB,1:500 - 1:2000 | IP,1:20 - 1:50

Background: This gene encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Extra Notes: Western blot analysis of extracts of various cell lines, using GPS2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug GPS2 antibody. Western blot was performed from the immunoprecipitate using GPS2 antibody at a dilution of 1:1000.

Bioworld Molecular Weight: 37kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: GPS2;AMF-1

Immunogen: A synthetic peptide of mouse GPS2(NP_062700.2).

Conjugate: Unconjugated

Modification: Unmodified