Product Description

GPx-4 Polyclonal Antibody

Catalogue Numbers: BS67575-50, BS67575-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P36969

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: The selenoprotein glutathione peroxidase 4 (GPX4) is a master regulator of ferroptosis, a form of programmed cell death induced by iron-dependent lipid peroxidation . GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis . Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons . In addition, some therapy-resistant cancer cells depend on GPX4 to survive. Loss of GPX4 leads to ferroptosis and thus prevents tumor relapse in mice . Furthermore, redox homeostasis mediated by GPX4 is essential for activation of the cytosolic DNA-sensing cGAS-STING pathway and initiation of the subsequent innate immune response .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GPx-4 protein.

Extra Notes: Western blot analysis of GPx-4 expression in HEK293T (A), K562 (B) whole cell lysates.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Phospholipid hydroperoxide glutathione peroxidase mitochondrial; PHGPx; Glutathione peroxidase 4; GPx-4; GSHPx-4

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPx-4. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified