GPX1 Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB9240-50, MB9240-100
Product: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P07203
Host: Mouse
Reactivity: Human
Applications: WB, ICC IHC FC
All Applications: WB:1:1,000
IHC:1:200
ICC:1:200
FC:1:100-1:200
Background: GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: GPX1 monoclonal antibody detects endogenous levels of GPX1 protein.
Bioworld Molecular Weight: 22 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes:
Positive control: Western blot analysis of GPX1 on different cell lysates using anti-GPX1 antibody at 1/1000 dilution.
Positive control:
Lane 1: THP-1
Lane 2: HepG2
Lane 3: 293T, ICC staining GPX1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: GSHPx-1, GPx-1, AL033363, Cellular glutathione peroxidase, Glutathione peroxidase 1, Glutathione peroxidase, GPx 1, GPx-1, GPX1, GPX1, GPXD, GSHPx-1, GSHPX1, MGC14399, MGC88245,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified