GPX1 Mouse Monoclonal Antibody-MB9027

GPX1 Mouse Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9027-50, MB9027-100

Product: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07203

Host: Mouse

Reactivity: Human

Applications: WB, ICC IHC FC

All Applications: WB: 1:1000
IHC/ICC: 1:200
FC: 1:100-1:200

Background: GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of GPX1 and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GPX1 Mouse mAb at 1:500 dilution
Lane 1: THP-1 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: 293T whole cell lysate, Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GPX1 antibody. Counter stained with hematoxylin., ICC staining GPX1 in Hela cells (red). =Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; GPX1

Immunogen: This antibody is produced by immunizing mice with a recombinant protein corresponding to a region of GPX1.

Conjugate: Unconjugated

Modification: Unmodified