Product Description

GPX1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90594-50, BS90594-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07203(Human) P11352(Mouse) P04041(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IP

All Applications: WB:1:1,000-1:2,000

Background: Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers. GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Glutathione Peroxidase 1 on differentLysates using anti-Glutathione Peroxidase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: THP-1

Bioworld Molecular Weight: 22 kDa

Specificity: GPX1 polyclonal antibody detects endogenous levels of GPX1 protein.

Alternative Name: GPx-1, GSHPx-1, GPX1, AL033363, Cellular glutathione peroxidase, Glutathione peroxidase 1, Glutathione peroxidase, GPx 1, GPx-1, GPX1, GPX1, GPXD, GSHPx-1, GSHPX1, MGC14399, MGC88245,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified